Deuterated Matrix-Assisted Laser Desorption Ionization Matrix Uncovers Masked Mass Spectrometry Imaging Signals of Small Molecules

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• 作者：Mohammadreza Shariatgorji ; Anna Nilsson ; Richard J. A. Goodwin ; Per Svenningsson ; Nicoletta Schintu ; Zoltan Banka ; Laszlo Kladni ; Tibor Hasko ; Andras Szabo ; Per E. Andren
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：August 21, 2012
• 年：2012
• 卷：84
• 期：16
• 页码：7152-7157
• 全文大小：311K
• 年卷期：v.84,no.16(August 21, 2012)
• ISSN：1520-6882

文摘

D⁴-伪-Cyano-4-hydroxycinnamic acid (D⁴-CHCA) has been synthesized for use as a matrix for matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) and MALDI-MS imaging (MSI) of small molecule drugs and endogenous compounds. MALDI-MS analysis of small molecules has historically been hindered by interference from matrix ion clusters and fragment peaks that mask signals of low molecular weight compounds of interest. By using D⁴-CHCA, the cluster and fragment peaks of CHCA, the most common matrix for analysis of small molecules, are shifted by + 4, + 8 and + 12 Da, which expose signals across areas of the previously concealed low mass range. Here, obscured MALDI-MS signals of a synthetic small molecule pharmaceutical, a naturally occurring isoquinoline alkaloid, and endogenous compounds including the neurotransmitter acetylcholine have been unmasked and imaged directly from biological tissue sections.