Oligonucleotide-Stabilized Ag Nanocluster Fluorophores

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• 作者：Chris I. Richards ; Sungmoon Choi ; Jung-Cheng Hsiang ; Yasuko Antoku ; Tom Vosch ; Angelo Bongiorno ; Yih-Ling Tzeng ; Robert M. Dickson
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：April 16, 2008
• 年：2008
• 卷：130
• 期：15
• 页码：5038 - 5039
• 全文大小：774K
• 年卷期：v.130,no.15(April 16, 2008)
• ISSN：1520-5126

文摘

Single-stranded oligonucleotides stabilize highly fluorescent Ag nanoclusters, with emission colors tunable via DNA sequence. We utilized DNA microarrays to optimize these scaffold sequences for creating nearly spectrally pure Ag nanocluster fluorophores that are highly photostable and exhibit great buffer stability. Five different nanocluster emitters have been created with tunable emission from the blue to the near-IR and excellent photophysical properties. Ensemble and single molecule fluorescence studies show that oligonucleotide encapsulated Ag nanoclusters exhibit significantly greater photostability and higher emission rates than commonly used cyanine dyes.