Rational Design of Chemical Ligands for Selective Mitochondrial Targeting
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- 作者：Silvie Rimpelov谩 ; Tom谩拧 B艡铆za ; Jarmila Kr谩lov谩 ; Kamil Z谩ruba ; Zden臎k Kej铆k ; Ivana C铆sa艡ov谩 ; Pavel Mart谩sek ; Tom谩拧 Ruml ; Vladim铆r Kr谩l
- 刊名：Bioconjugate Chemistry
- 出版年：2013
- 出版时间：September 18, 2013
- 年：2013
- 卷：24
- 期：9
- 页码：1445-1454
- 全文大小：384K
- 年卷期：v.24,no.9(September 18, 2013)
- ISSN：1520-4812
文摘
The rational design of molecules with selective intracellular targeting is a great challenge for contemporary chemistry and life sciences. Here, we demonstrate a rational approach to development of compartment-specific fluorescent dyes from the 纬-aryl substituted pentamethine family. These novel dyes exhibit an extraordinary affinity and selectivity for cardiolipin in inner mitochondrial membrane and possess excellent photostability, fluorescent properties, and low phototoxicity. Selective imaging of live and fixed mitochondria was achieved in various cell lines using nanomolar concentrations of these dyes. Their high localization specificity and low toxicity enables study of morphological changes, structural complexity, and dynamics of mitochondria playing a pivotal role in many pathological diseases. These far-red emitting dyes could also serve in a variety of biomedical applications.