A Universal Nucleoside with Strong Two-Band Switchable Fluorescence and Sensitivity to the Environment for Investigating DNA Interactions
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- 作者：Dmytro Dziuba ; Viktoriia Y. Postupalenko ; Marie Spadafora ; Andrey S. Klymchenko ; Vincent Gu茅rineau ; Yves M茅ly ; Rachid Benhida ; Alain Burger
- 刊名：The Journal of the American Chemical Society
- 出版年：2012
- 出版时间：June 20, 2012
- 年：2012
- 卷：134
- 期：24
- 页码：10209-10213
- 全文大小：267K
- 年卷期：v.134,no.24(June 20, 2012)
- ISSN：1520-5126
文摘
With the aim of developing a new tool to investigate DNA interactions, a nucleoside analogue incorporating a 3-hydroxychromone (3HC) fluorophore as a nucleobase mimic was synthesized and incorporated into oligonucleotide chains. In comparison with existing fluorescent nucleoside analogues, this dye features exceptional environmental sensitivity switching between two well-resolved fluorescence bands. In labeled DNA, this nucleoside analogue does not alter the duplex conformation and exhibits a high fluorescence quantum yield. This probe is up to 50-fold brighter than 2-aminopurine, the fluorescent nucleoside standard. Moreover, the dual emission is highly sensitive to the polarity of the environment; thus, a strong shielding effect of the flanking bases from water was observed. With this nucleoside, the effect of a viral chaperone protein on DNA base stacking was site-selectively monitored.