Manufacture and Properties of Bifidogenic Saccharides Derived from Wood Mannan
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- 作者：Sandra Rivas ; Beatriz Gull贸n ; Patricia Gull贸n ; Jos茅 Luis Alonso ; Juan Carlos Paraj贸
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2012
- 出版时间：May 2, 2012
- 年：2012
- 卷：60
- 期：17
- 页码：4296-4305
- 全文大小：317K
- 年卷期：v.60,no.17(May 2, 2012)
- ISSN：1520-5118
文摘
Pinus pinaster wood samples were subjected to double hydrothermal processing. The liquors coming from the second stage, containing soluble saccharides of polymeric or oligomeric nature from hemicelluloses (POHs), were subjected to membrane processing (operating in discontinuous diafiltration) for refining and fractionation. Refined POH fractions were characterized by matrix-assisted laser desorption/ionization time of flight mass spectrometry and chromatographic techniques. The most complex POH component was made up of 14 hexoses and contained 4 acetyl groups. The fermentability of purified POHs by human fecal inocula was assessed by measuring both carbon source consumption and formation of short-chain fatty acids. The bifidogenic ability of POHs was confirmed by fluorescence in situ hybridization. The stimulatory effects on the bifidobacterial population reached by POHs were of the same order as those obtained with commercial fructooligosaccharides.