Effect of Postharvest Storage on the Expression of the Apple Allergen Mal d 1

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• 作者：Ana I. Sancho ; Robert Foxall ; Tom Browne ; Rickmer Dey ; Laurian Zuidmeer ; Gorji Marzban ; Keith W. Waldron ; Ronald van Ree ; Karin Hoffmann-Sommergruber ; Margit Laimer ; and ; E. N. Clare Mills
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：August 9, 2006
• 年：2006
• 卷：54
• 期：16
• 页码：5917 - 5923
• 全文大小：256K
• 年卷期：v.54,no.16(August 9, 2006)
• ISSN：1520-5118

文摘

Consumption of fresh apples can cause allergy in susceptible individuals. A competitive enzyme-linked immunosorbent assay (ELISA) has been developed to determine Mal d 1 levels in apple pulpusing a monoclonal antibody (BIP-1). The ELISA was able to rank ten cultivars according to theirMal d 1 content (between 3.8 and 72.5 ages/entities/mgr.gif">g/g pulp). For the first time, it has been demonstrated thatgrowing conditions and postharvest storage, using three different treatments over a 5 month periodin 2 consecutive years, increase Mal d 1 expression at a translational and transcriptional level (3.5-and 8.5-fold under controlled atmosphere storage). Expression of three major Mal d 1 isoforms wasobserved by real-time polymerase chain reaction over the 5 month storage period, and Mal d 1.02was the most highly expressed isoform. In conclusion, Mal d 1 gene expression was significantlyincreased during modified atmosphere storage. Individuals suffering from birch pollen-apple allergysyndrome might experience fewer problems consuming freshly picked apples.