Consumption of fresh
apples c
an c
ause
allergy in susceptible individu
als. A competitive enzyme-linked immunosorbent
ass
ay (ELISA) h
as been developed to determine M
al d 1 levels in
apple pulpusing
a monoclon
al
antibody (BIP-1). The ELISA w
as
able to r
ank ten cultiv
ars
according to theirM
al d 1 content (between 3.8
and 72.5
![](/im<font color=)
ages/entities/mgr.gif">g/g pulp). For the first time, it h
as been demonstr
ated th
atgrowing conditions
and posth
arvest stor
age, using three different tre
atments over
a 5 month periodin 2 consecutive ye
ars, incre
ase M
al d 1 expression
at
a tr
ansl
ation
al
and tr
anscription
al level (3.5-
and 8.5-fold under controlled
atmosphere stor
age). Expression of three m
ajor M
al d 1 isoforms w
asobserved by re
al-time polymer
ase ch
ain re
action over the 5 month stor
age period,
and M
al d 1.02w
as the most highly expressed isoform. In conclusion, M
al d 1 gene expression w
as signific
antlyincre
ased during modified
atmosphere stor
age. Individu
als suffering from birch pollen-
apple
allergysyndrome might experience fewer problems consuming freshly picked
apples.