Maturity and Storage Influence on the Apple (Malus domestica) Allergen Mal d 3, a Nonspecific Lipid Transfer Protein

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• 作者：Ana I. Sancho ; Robert Foxall ; Neil M. Rigby ; Thomas Browne ; Laurian Zuidmeer ; Ronald van Ree ; Keith W. Waldron ; and E. N. Clare Mills
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：July 12, 2006
• 年：2006
• 卷：54
• 期：14
• 页码：5098 - 5104
• 全文大小：162K
• 年卷期：v.54,no.14(July 12, 2006)
• ISSN：1520-5118

文摘

Consumption of apples can provoke severe allergic reactions, in susceptible individuals, due to thepresence of the allergen Mal d 3, a nonspecific lipid transfer protein, found largely in the fruit skin.Levels of Mal d 3 were determined in peel as a function of apple cultivar, position of the fruit growingon the tree, apple maturity, and postharvest storage by ELISA. As the apples mature, Mal d 3 levelsincreased, although the rate was dependent on cultivar and tree position. During storage, levels ofMal d 3 decreased in all cultivars (cvs. Cox, Jonagored, and Gala), the rate of overall decreasebeing greatest under controlled atmosphere conditions. There was no correlation between Mal d 3levels and total apple peel protein, indicating specific alterations in Mal d 3 expression. Thus pre-and postharvest treatments (i.e., storage) can modify the allergen load in apple peel, the highestlevels being found in overly mature and freshly harvested fruits.Keywords: Apple; allergens; nonspecific lipid transfer protein; Mal d 3; cultivar; storage; maturity;nsLTP; Mal d 3 expression