Proteome Cataloging and Relative Quantification of Francisella tularensis tularensis Strain Schu4 in 2D PAGE Using Preparative Isoelectric Focusing

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• 作者：Robert H. Waldo ; Edwin D. Cummings ; Siva T. Sarva ; Janet M. Brown ; Crystal M. Lauriano ; Lorne A. Rose ; Robert J. Belland ; Karl E. Klose ; George M. Hilliard
• 刊名：Journal of Proteome Research
• 出版年：2007
• 出版时间：September 2007
• 年：2007
• 卷：6
• 期：9
• 页码：3484 - 3490
• 全文大小：407K
• 年卷期：v.6,no.9(September 2007)
• ISSN：1535-3907

文摘

The protein complement of whole cell extract of the bacterium Francisella tularensis tularensis wasanalyzed using two-dimensional electrophoresis with preparative isoelectric focusing in the firstdimension. The format allows the quantification of relative protein abundance by linear densitometryand extends the potential dynamic range of protein detection by as much as an order of magnitude.The relative abundance and rank order of 136 unique proteins identified in F. tularensis tularensis wereestablished. It is estimated that 16% of the moderately to highly expressed proteins and 8% of allpredicted non-pseudogenes were identified by comparing this proteome information with the relativeabundance of mRNA as measured by microarray. This rank-ordered proteome list provides an importantresource for understanding the pathogenesis of F. tularensis and is a tool for the selection and designof synthetic vaccines. This method represents a useful additional technique to improve whole proteomeanalyses of simple organisms.