Elevated Levels of Phosphorylated Fibrinogen--Isoforms and Differential Expression of Other Post-Translationally Modified Proteins i
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- 作者：Yuko Ogata ; Carrie J. Hepplmann ; M. Cristine Charlesworth ; Benjamin J. Madden ; Melinda N. Miller ; Kimberly R. Kalli ; William A. Cilby ; H. Robert Bergen III ; Diana A. Saggese ; David C. Muddiman
- 刊名：Journal of Proteome Research
- 出版年：2006
- 出版时间：December 2006
- 年：2006
- 卷：5
- 期：12
- 页码：3318 - 3325
- 全文大小：462K
- 年卷期：v.5,no.12(December 2006)
- ISSN：1535-3907
文摘
We evaluated the differentially expressed proteins in the plasma of ovarian cancer (OVC) patients using2-D SDS-polyacrylamide gel electrophoresis (SDS-PAGE) with post-translational modification (PTM)specific stains after the removal of six high-abundance proteins. The pooled plasma from patients withstage III or IV OVC was compared to a pooled postmenopausal age-matched control. Several proteinswere identified as differentially expressed in the plasma of OVC patients. Among them, the phosphorylated fibrinogen-
-chain isoform (containing fibrinopeptide-A) was found to be up-regulated.Previously in our laboratory, phosphorylated fibrinopeptide-A was found to be up-regulated in the lowmolecular weight fraction of serum derived from OVC patients. We examined the levels of phosphorylated fibrinogen--chain in each patient that constituted the pooled plasma using Western blot,mass spectrometry (MS), and PTM specific stains. Phosphoprotein bands containing fibrinogen--chainfragments showed up-regulation in all OVC patients.
-chain isoform (containing fibrinopeptide-A) was found to be up-regulated.Previously in our laboratory, phosphorylated fibrinopeptide-A was found to be up-regulated in the lowmolecular weight fraction of serum derived from OVC patients. We examined the levels of phosphorylated fibrinogen--chain in each patient that constituted the pooled plasma using Western blot,mass spectrometry (MS), and PTM specific stains. Phosphoprotein bands containing fibrinogen--chainfragments showed up-regulation in all OVC patients.