A Europium Complex That Selectively Stains Nucleoli of Cells
详细信息 查看全文
- 作者：Junhua Yu ; David Parker ; Robert Pal ; Robert A. Poole ; and Martin J. Cann
- 刊名：Journal of the American Chemical Society
- 出版年：2006
- 出版时间：February 22, 2006
- 年：2006
- 卷：128
- 期：7
- 页码：2294 - 2299
- 全文大小：167K
- 年卷期：v.128,no.7(February 22, 2006)
- ISSN：1520-5126
文摘
A europium complex selectively staining the nucleolus of NIH 3T3, HeLa, and HDF cells isreported. This complex possesses not only the advantage of the long lifetime of europium emission (0.3ms), but also a chromophore that allows excitation at a relatively long wavelength (
max = 384 nm) andgives rise to an acceptable quantum yield (9%). The complex can be used both in live cell and fixed cellimaging, giving an average intracellular concentration on the order of 0.5 
M. Strong binding to serumalbumin has been demonstrated by examination of the analogous gadolinium complex, studying relaxivitychanges with increasing protein concentration. The intracellular speciation of the complex has been examinedby circularly polarized emission spectroscopy and is consistent with the presence of more than one europiumspecies, possibly protein bound.
max = 384 nm) andgives rise to an acceptable quantum yield (9%). The complex can be used both in live cell and fixed cellimaging, giving an average intracellular concentration on the order of 0.5 M. Strong binding to serumalbumin has been demonstrated by examination of the analogous gadolinium complex, studying relaxivitychanges with increasing protein concentration. The intracellular speciation of the complex has been examinedby circularly polarized emission spectroscopy and is consistent with the presence of more than one europiumspecies, possibly protein bound.