A one-step simple synthesis of silver colloid nanoparticles with controllable sizes is presented. In this synthesis,reduction of [Ag(NH
3)
2]
+ complex cation by four saccharides was performed. Four saccharides were used:two monosaccharides (glucose and galactose) and two disaccharides (maltose and lactose). The synthesesperformed at various ammonia concentrations (0.005-0.20 mol L
-1) and pH conditions (11.5-13.0) produceda wide range of particle sizes (25-450 nm) with narrow size distributions, especially at the lowest ammoniaconcentrations. The average size, size distribution, morphology, and structure of particles were determinedby dynamic light scattering (DLS), transmission electron microscopy (TEM), and UV/Visible absorptionspectrophotometry. The influence of the saccharide structure (monosacharides versus disaccharides) on thesize of silver particles is briefly discussed. The reduction of [Ag(NH
3)
2]
+ by maltose produced silver particleswith a narrow size distribution with an average size of 25 nm, which showed high antimicrobial and bactericidalactivity against Gram-positive and Gram-negative bacteria, including highly multiresistant strains such asmethicillin-resistant
Staphylococcus aureus. Antibacterial activity of silver nanoparticles was found to bedependent on the size of silver particles. A very low concentration of silver (as low as 1.69
![](/images/entities/mgr.gif)
g/mL Ag) gaveantibacterial performance.