In this first systematic study, we describe the influence of various surfactants and polymers on aggregationstability and antibacterial activity of silver nanoparticles (NPs) prepared by a modified Tollens process. Thesurfactant/polymer stabilizing effect was monitored using the newly established method based on a titrationof the aqueous dispersion of the silver NPs by aqueous solution of poly(diallyldimethylammonium) chloride(PDDA). The aggregation process was evaluated by the dynamic light scattering (DLS) and UV/vis spectrameasurements and finally confirmed by TEM. Among all of the investigated modifiers, two surfactants (sodiumdodecyl sulfate-SDS and polyoxyethylenesorbitane monooleate-Tween 80) and one polymer (polyvinylpyrrolidone-PVP 360) exhibit superior stabilization of the silver NP dispersions against the process of aggregation.The differences in the stabilization ability of various tested substances are discussed with respect to theirstructure and possible mechanism of the surface interaction with the NPs. The antibacterial activity of themodified silver NPs was significantly enhanced especially when modified by SDS where the minimuminhibition concentration (MIC) decreased under the "magical value" of 1
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g·mL
-1. A correlation was foundbetween the aggregation stability and enhanced antibacterial activity in the system of the silver NPs modifiedby SDS, Tween 80, and high molecular polymer PVP 360.