Active Release of Nitric Oxide-Releasing Dendrimers from Electrospun Polyurethane Fibers

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• 作者：Brittany V. Worley ; Robert J. Soto ; Paige C. Kinsley ; Mark H. Schoenfisch
• 刊名：ACS Biomaterials Science & Engineering
• 出版年：2016
• 出版时间：March 14, 2016
• 年：2016
• 卷：2
• 期：3
• 页码：426-437
• 全文大小：550K
• ISSN：2373-9878

文摘

The fabrication of electrospun composite polyurethane fibers capable of dual-action antibacterial dendrimer release is reported. Generation 4 (G4) poly(amidoamine) dendrimers were functionalized with octyl alkyl chain or quaternary ammonium (QA) moieties followed by modification of the resulting secondary amines with N-diazeniumdiolate nitric oxide (NO) donors to produce dual-action antibacterial dendrimers. Control and NO-releasing dendrimers were doped into polyurethane solutions prior to electrospinning of the polymer to yield well-defined dendrimer-doped composite polyurethane fibers. The fiber mats were semiporous (≥30% porosity) and exhibited high water uptake (>100% relative to fiber mass). Dendrimer- and NO-release characteristics (rates and totals) were dependent on the dendrimer modification and polyurethane composition, with total dendrimer- and NO-release amounts ranging from 10–80 μg/mg and 0.027–0.072 μmol NO/mg, respectively. The antibacterial action of the fibers was evaluated against Gram-negative and Gram-positive bacterial strains. Nitric oxide-releasing fibers demonstrated broad-spectrum bactericidal action at short (2 h) and long (24 h) time scales.