Protein and Steroid Profiles in Follicular Fluid after Ovarian Hyperstimulation as Potential Biomarkers of IVF Outcome
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- 作者：Mark M Kushnir ; Tord Naess茅n ; Kjell Wanggren ; Alan L Rockwood ; David K Crockett ; Jonas Bergquist
- 刊名：Journal of Proteome Research
- 出版年：2012
- 出版时间：October 5, 2012
- 年：2012
- 卷：11
- 期：10
- 页码：5090-5100
- 全文大小：288K
- 年卷期：v.11,no.10(October 5, 2012)
- ISSN：1535-3907
文摘
Controlled ovarian hyperstimulation is performed to assist with generation of multiple mature oocytes for use in in vitro fertilization (IVF). The goal of our study was to evaluate differences in protein and steroid profiles in ovarian follicular fluid (hFF) samples obtained during oocyte retrieval from women undergoing IVF treatment and to identify physiological pathways associated with the proteins. The hFF samples were depleted of abundant proteins, fractionated by ultrafiltration, digested, and analyzed by nano-LC鈥換TOF. Concentrations of 15 endogenous steroids were determined in the samples using LC鈥揗S/MS methods. The total number of proteins identified in the samples was 75, of which 4, 7, and 2 were unique to the samples from women with viable pregnancy, miscarriage, and no pregnancy, respectively. Identified proteins were associated with the acute response signaling, coagulation system, intrinsic and extrinsic prothrombin activation, complement system, neuroprotective role of THOP1, FXR/RXR activation, role of tissue factor, and growth hormone pathways. A greater number of proteins associated with biosynthesis was found in hFF samples corresponding to the oocytes resulting in pregnancy. The abundance of seven proteins was found to be associated with steroidogenesis. The obtained data will contribute to better understanding of the pathogenesis and development of noninvasive markers for assessment of oocytes viability.