Designing Two Self-Assembly Mechanisms into One Viral Capsid Protein

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• 作者：Mark B. van Eldijk ; Joseph C.-Y. Wang ; Inge J. Minten ; Chenglei Li ; Adam Zlotnick ; Roeland J. M. Nolte ; Jeroen J. L. M. Cornelissen ; Jan C. M. van Hest
• 刊名：The Journal of the American Chemical Society
• 出版年：2012
• 出版时间：November 14, 2012
• 年：2012
• 卷：134
• 期：45
• 页码：18506-18509
• 全文大小：275K
• 年卷期：v.134,no.45(November 14, 2012)
• ISSN：1520-5126

文摘

ELP-CP, a structural fusion protein of the thermally responsive elastin-like polypeptide (ELP) and a viral capsid protein (CP), was designed, and its assembly properties were investigated. Interestingly, this protein-based block copolymer could be self-assembled via two mechanisms into two different, well-defined nanocapsules: (1) pH-induced assembly yielded 28 nm virus-like particles, and (2) ELP-induced assembly yielded 18 nm virus-like particles. The latter were a result of the emergent properties of the fusion protein. This work shows the feasibility of creating a self-assembly system with new properties by combining two structural protein elements.