Biosynthesis of Isoprenoids. A Rapid Method for the Preparation of Isotope-Labeled 4-Diphosphocytidyl-2C-methyl-D-erythritol

详细信息    查看全文

• 作者：Felix Rohdich ; Christoph A. Schuhr ; Stefan Hecht ; Stefan Herz ; Juraithip Wungsintaweekul ; Wolfgang Eisenreich ; Meinhart H. Zenk ; and Adelbert Bacher
• 刊名：Journal of the American Chemical Society
• 出版年：2000
• 出版时间：October 11, 2000
• 年：2000
• 卷：122
• 期：40
• 页码：9571 - 9574
• 全文大小：68K
• 年卷期：v.122,no.40(October 11, 2000)
• ISSN：1520-5126

文摘

4-Diphosphocytidyl-2C-methyl-D-erythritol serves as an intermediate in the nonmevalonate pathwayof isoprenoid biosynthesis. The compound has been prepared in millimole quantity by a sequence of one-potreactions using ¹³C-labeled pyruvate and dihydroxyacetone phosphate or ¹³C-labeled glucose as starting materialsand recombinant enzymes of the nonmevalonate isoprenoid pathway as catalysts. The method has been usedfor the preparation of various 4-diphosphocytidyl-2C-methyl-D-erythritol isotopomers in high yield.