Tailoring the Trajectory of Cell Rolling with Cytotactic Surfaces

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• 作者：Collin Edington ; Hironobu Murata ; Richard Koepsel ; Jill Andersen ; Sungeun Eom ; Takeo Kanade ; Anna C. Balazs ; German Kolmakov ; Carsen Kline ; Daniel McKeel ; Zvi Liron ; Alan J. Russell
• 刊名：Langmuir
• 出版年：2011
• 出版时间：December 20, 2011
• 年：2011
• 卷：27
• 期：24
• 页码：15345-15351
• 全文大小：885K
• 年卷期：v.27,no.24(December 20, 2011)
• ISSN：1520-5827

文摘

Cell separation technology is a key tool for biological studies and medical diagnostics that relies primarily on chemical labeling to identify particular phenotypes. An emergent method of sorting cells based on differential rolling on chemically patterned substrates holds potential benefits over existing technologies, but the underlying mechanisms being exploited are not well characterized. In order to better understand cell rolling on complex surfaces, a microfluidic device with chemically patterned stripes of the cell adhesion molecule P-selectin was designed. The behavior of HL-60 cells rolling under flow was analyzed using a high-resolution visual tracking system. This behavior was then correlated to a number of established predictive models. The combination of computational modeling and widely available fabrication techniques described herein represents a crucial step toward the successful development of continuous, label-free methods of cell separation based on rolling adhesion.