Fengycin C Produced by Bacillus subtilis EA-CB0015

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• 作者：Valeska Villegas-Escobar ; Isabel Ceballos ; John J. Mira ; Luz Edith Argel ; Sergio Orduz Peralta ; Magally Romero-Tabarez
• 刊名：Journal of Natural Products
• 出版年：2013
• 出版时间：April 26, 2013
• 年：2013
• 卷：76
• 期：4
• 页码：503-509
• 全文大小：469K
• 年卷期：v.76,no.4(April 26, 2013)
• ISSN：1520-6025

文摘

<i>Bacillus subtilisi> EA-CB0015 was isolated from the phyllosphere of a banana plant and tested for its potential to produce bioactive compounds against <i>Mycosphaerella fijiensis.i> Using a dual plate culture technique the cell-free supernatant of <i>B. subtilisi> EA-CB0015 produced inhibition values of 89 卤 1%. The active compounds were purified by solid-phase extraction and HPLC, and their primary structures determined using mass spectrometry and amino acid analysis. A new fengycin isoform, fengycin C, with the amino acid sequence Glu-Orn-Tyr-Thr-Glu-Val-Pro-Gln-Thr-Ile was isolated. The peptidic moiety differs from fengycin B at position 9 and from fengycin A at positions 6 and 9. The 尾-hydroxy fatty acyl chain is connected to the N-terminal of the decapeptide and can be saturated or unsaturated, ranging from 14 to 18 carbons. The C-terminal residue of the peptidic moiety is linked to the tyrosine residue at position 3, forming the branching point of the acyl peptide and the eight-membered cyclic lactone.