Site-Specific Protein Immobilization by Staudinger Ligation

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• 作者：Matthew B. Soellner ; Kimberly A. Dickson ; Bradley L. Nilsson ; and Ronald T. Raines
• 刊名：Journal of the American Chemical Society
• 出版年：2003
• 出版时间：October 1, 2003
• 年：2003
• 卷：125
• 期：39
• 页码：11790 - 11791
• 全文大小：76K
• 年卷期：v.125,no.39(October 1, 2003)
• ISSN：1520-5126

文摘

The Staudinger ligation between an azido-protein and a phosphinothioester-derivatized surface is demonstrated to be an effective means for the site-specific, covalent immobilization of a protein. Immobilization yields of >50% are obtained in <1 min, and immobilized proteins have >80% of their expected activity. No other method enables more rapid immobilization or a higher yield of active protein. Because azido-peptides and azido-proteins are readily attainable by synthesis, biosynthesis, or semisynthesis, the Staudinger ligation could be of unsurpassed utility in creating microarrays of functional peptides and proteins.