Rapid Determination of Hydrogen Peroxide Using Peroxidase Immobilized on Amberlite IRA-743 and Minerals in Honey
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- 作者：Rô ; mulo Augusto de Abreu Franchini ; Cabrini Ferraz de Souza ; Rosana Colombara ; Maria Auxiliadora Costa Matos ; Renato Camargo Matos
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：August 22, 2007
- 年：2007
- 卷：55
- 期：17
- 页码：6885 - 6890
- 全文大小：80K
- 年卷期：v.55,no.17(August 22, 2007)
- ISSN：1520-5118
文摘
Hydrogen peroxide and trace metals (K+, Ca2+, Na+, Mg2+, Mn2+, and Li+) were determined in 14samples of Brazilian commercial honeys. The method for the determination of H2O2 is based onselective oxidation of H2O2 using an on-line tubular reactor containing peroxidase immobilized onAmberlite IRA-743 resin. Reactors presented high stability for at least 2 weeks under intense use.The results show a simple, accurate, selective, and readily applied method to the determination ofH2O2 in honey. The trace metals were determined by capillary zone eletrophoresis. Mean contentsof 656, 69.1, 71.8, 36.0, 21.4, and 1.70 mg/kg were found, respectively, for K+, Ca2+, Na+, Mg2+,Mn2+, and Li+ in the analyzed honeys. The cations were identified by comparison of the relativemigration times of their peaks with the Ba2+ migration time used as reference. The electrophoreticanalysis was simple and rapid and did not require any other preparation of sample than dilution andfiltration.