Arsenic-Rich Acid Mine Water with Extreme Arsenic Concentration: Mineralogy, Geochemistry, Microbiology, and Environmental Implications

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• 作者：Juraj Majzlan ; Jakub Pl谩拧il ; Radek 艩koda ; Johannes Gescher ; Felix K枚gler ; Anna Rusznyak ; Kirsten K眉sel ; Thomas R. Neu ; Stefan Mangold ; J枚rg Rothe
• 刊名：Environmental Science & Technology
• 出版年：2014
• 出版时间：December 2, 2014
• 年：2014
• 卷：48
• 期：23
• 页码：13685-13693
• 全文大小：344K
• ISSN：1520-5851

文摘

Extremely arsenic-rich acid mine waters have developed by weathering of native arsenic in a sulfide-poor environment on the 10th level of the Svornost mine in J谩chymov (Czech Republic). Arsenic rapidly oxidizes to arsenolite (As₂O₃), and there are droplets of liquid on the arsenolite crust with high As concentration (80,000鈥?30,000 mg路L^鈥?), pH close to 0, and density of 1.65 g路cm^鈥?. According to the X-ray absorption spectroscopy on the frozen droplets, most of the arsenic is As(III) and iron is fully oxidized to Fe(III). The EXAFS spectra on the As K edge can be interpreted in terms of arsenic polymerization in the aqueous solution. The secondary mineral that precipitates in the droplets is kaatialaite [Fe³⁺(H₂AsO₄)₃路5H₂O]. Other unusual minerals associated with the arsenic lens are b臎hounekite [U⁴⁺(SO₄)₂路4H₂O], 拧t臎pite [U⁴⁺(AsO₃OH)₂路4H₂O], vysok媒ite [U⁴⁺[AsO₂(OH)₂]₄路4H₂O], and an unnamed phase (H₃O)⁺₂(UO₂)₂(AsO₄)₂路nH₂O. The extremely low cell densities and low microbial biomass have led to insufficient amounts of DNA for downstream polymerase chain reaction amplification and clone library construction. We were able to isolate microorganisms on oligotrophic media with pH 鈭?1.5 supplemented with up to 30 mM As(III). These microorganisms were adapted to highly oligotrophic conditions which disabled long-term culturing under laboratory conditions. The extreme conditions make this environment unfavorable for intensive microbial colonization, but our first results show that certain microorganisms can adapt even to these harsh conditions.