文摘
Low-protein-fouling poly(ethylene glycol) (PEG-like) plasma polymer films were prepared using radio frequencyglow discharge polymerization of diethylene glycol dimethyl ether (DGpp) on top of a heptylamine plasma polymerprimer layer. By varying the plasma deposition conditions, the chemistry of the DGpp film was influenced, especiallyin regard to the level of ether content, which in turn influenced the relative levels of bovine serum albumin andlysozyme protein fouling. Surface potential measurements indicated that these surfaces carried a net negative charge.While protein fouling remained low (~10 ng/cm2), there was a slightly higher level of the positively charged proteinadsorbed on these films than the negative protein. The interaction forces measured between a silica spherical surfaceon both "high"- and "low"-protein-fouling DGpp films were all repulsive and short ranged (2-3 nm). There was nocorrelation between the surface forces measured for high- and low-protein-fouling DGpp films. Thus, it appears thatenthalpic effects are very important in reducing protein adsorption. We therefore conclude that it is the concentrationof residual, ethylene glycol containing species that are the crucial parameter determining protein resistance due toa combination of both entropic and enthalpic effects.