Technique for Screening Immune-Enhancing Polysaccharides in Food Using 1,25-Dihydroxyvitamin D3-Differentiated HL60 Cells
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- 作者：Manami Monobe ; Kaori Ema ; Fumiko Kato ; Hisako Hirokane ; Mari Maeda-Yamamoto
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2007
- 出版时间：April 4, 2007
- 年：2007
- 卷：55
- 期：7
- 页码：2543 - 2547
- 全文大小：380K
- 年卷期：v.55,no.7(April 4, 2007)
- ISSN：1520-5118
文摘
A technique for screening immune-enhancing polysaccharides in food using the phagocytotic activityof 1,25-dihydroxyvitamin D3 (VD3)-differentiated HL60 cells is presented. HL60 cells, a human acutepromyelocytic cell line, can differentiate along the monocytic lineage following exposure to VD3 orphorbol-12-myristate-13-acetate (PMA). For differentiated cells along the monocytic pathway, HL60cells were maintained in RPMI 1640 medium supplemented with 10% FBS and 120 nM VD3 formore than 1 week. VD3-differentiated HL60 cells were seeded into 48-well plates, YG-labeledmicrospheres and polysaccharides were added and mixed using a plate shaker at 1100 rpm for 30s, and then the mixture was incubated overnight at 37 
C in 5% CO2. The cells were fixed with 2%formaldehyde and resuspended in phosphate-buffered saline. The rate of phagocytosis was measuredwith a flow cytometer. VD3-differentiated cells but not non- and PMA-differentiated cells resulted inan elevation of phagocytic activity by various immune-enhancing polysaccharides in foods.
C in 5% CO2. The cells were fixed with 2%formaldehyde and resuspended in phosphate-buffered saline. The rate of phagocytosis was measuredwith a flow cytometer. VD3-differentiated cells but not non- and PMA-differentiated cells resulted inan elevation of phagocytic activity by various immune-enhancing polysaccharides in foods.