Bio-orthogonal Immobilization of Fibroblast Growth Factor 2 for Spatial Controlled Cell Proliferation

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• 作者：Tessa Lühmann ; Gabriel Jones ; Marcus Gutmann ; Jens-Christoph Rybak ; Joachim Nickel ; Marina Rubini ; Lorenz Meinel
• 刊名：ACS Biomaterials Science & Engineering
• 出版年：2015
• 出版时间：September 14, 2015
• 年：2015
• 卷：1
• 期：9
• 页码：740-746
• 全文大小：405K
• ISSN：2373-9878

文摘

Presentation of therapeutic proteins on material surfaces is challenged by random immobilization chemistries through lysine or cysteine residues, typically leading to heterogeneous product outcome. Pharmaceutical quality standards warrant a controlled process ideally through site specific conjugation. Therefore, we deployed genetic codon expansion to engineer a propargyl-mallcaps">l-lysine (Plk)-modified FGF-2 analogue, enabling site-specific copper(I)-catalyzed azide alkyne cycloaddition (CuAAC). Site-specific decoration of Plk-FGF-2 to particles sparked cell proliferation of human osteosarcoma cells in a spatially controlled manner around the decorated carrier, rendering this approach instrumental for the future design of quality-improved bioinstructive scaffold outcome.