Liquid Chromatography鈥揟andem Mass Spectrometry-Based Plasma Metabonomics Delineate the Effect of Metabolites鈥?Stability on Reliability of Potential Biomarkers

详细信息    查看全文

• 作者：Wei Yang ; Yanhua Chen ; Cong Xi ; Ruiping Zhang ; Yongmei Song ; Qimin Zhan ; Xiaofeng Bi ; Zeper Abliz
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：March 5, 2013
• 年：2013
• 卷：85
• 期：5
• 页码：2606-2610
• 全文大小：343K
• 年卷期：v.85,no.5(March 5, 2013)
• ISSN：1520-6882

文摘

Metabonomics is an important platform for investigating the metabolites of integrated living systems and their dynamic responses to changes caused by both endogenous and exogenous factors. A metabonomics strategy based on liquid chromatography鈥搈ass spectrometry/mass spectrometry in both positive and negative ion modes was applied to investigate the short-term and long-term stability of metabolites in plasma. Principal components analysis and ten types of identified metabolites were used to summarize the time-dependent change rules in metabolites systematically at different temperatures. The long-term stability of metabolites in plasma specimens stored at 鈭?0 掳C for five years was also studied. Analysis of these subjects identified 36 metabolites with statistically significant changes in expression (p < 0.05) and found a kind of metabolite with a hundred-fold change. The stability of metabolites in blood at 4 掳C for 24 h was also investigated. These studies show that a thorough understanding of the effects of metabolite stability are necessary for improving the reliability of potential biomarkers.