Dean Vortex Membrane Microfiltration and Diafiltration of rBDNF E. coli Inclusion Bodies

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• 作者：Maarten Schutyser ; Randall Rupp ; Janusz Wideman ; and Georges Belfort
• 刊名：Biotechnology Progress
• 出版年：2002
• 出版时间：April 2002
• 年：2002
• 卷：18
• 期：2
• 页码：322 - 329
• 全文大小：255K
• 年卷期：v.18,no.2(April 2002)
• ISSN：1520-6033

文摘

Cross-flow microfiltration (CMF) and diafiltration were used to concentrate and purifyrecombinant Brain-Derived Neutrophic Factor (rBDNF) inclusion bodies from an E.coli cell suspension and a homogenized E. coli cell suspension (homogenate/lysate).Although these processes have been tested industrially in pilot scale with conventionallinear membrane microfiltration modules, their performances were severely limiteddue to membrane fouling. The purpose of this work was to determine whether Deanvortex microfiltration with controlled centrifugal instabilities (Dean vortices producedin helical flow) could be used to improve filtration performance over that observedwith conventional linear cross-flow microfiltration (CMF). For the microfiltrationexperiments with the feeds containing cell and homogenate suspensions, improvementsin flux of about 50 and 70%, respectively, were obtained with the helical module ascompared with that obtained with the linear module. For diafiltration with thehomogenate suspension as feed, solute transport (as measured by mass) was from100 to 40% higher after 40 and 100 min, respectively, with the helical module ascompared with that obtained with the linear module. In the presence of the neutralsurfactant, Tween 20, solute transport for diafiltration was at least 25 times higherduring the first 10 min of operation and 100% higher after 300 min with the helicalmodule as compared with that obtained with the linear module. Clearly, improvedfiltration performance, a purer and more concentrated product, and substantial savingscan be expected with the new Dean vortex filters.