Low-Dose Responses to 2,3,7,8-Tetrachlorodibenzo-p-dioxin in Single Living Human Cells Measured by Synchrotron Infrared Spectromicroscopy

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• 作者：Hoi-Ying N. Holman ; Regine Goth-Goldstein ; Michael C. Martin ; Marion L. Russell ; and Wayne R. McKinney
• 刊名：Environmental Science & Technology
• 出版年：2000
• 出版时间：June 15, 2000
• 年：2000
• 卷：34
• 期：12
• 页码：2513 - 2517
• 全文大小：137K
• 年卷期：v.34,no.12(June 15, 2000)
• ISSN：1520-5851

文摘

Synchrotron radiation (SR)-based Fourier transforminfrared (FTIR) spectromicroscopy measurements arepresented of HepG2 cells exposed to an environmentalcontaminant that is a known ligand for the aryl hydrocarbon(Ah) receptor. Measurements were made on cellstreated with an Ah receptor-binding model compound 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). Compared to untreatedcontrol cells, treated cells displayed unique spectralchanges with TCDD concentrations of 0.01-10.0 nM. Keyspectral changes involved P=O, C-O, and C-H stretchingbands. The first changes are related to the environment ofthe phosphate backbone of nucleic acids. The C-Hstretching bands data show a relative increase in thenumber of methyl to methylene groups. An excellentcorrelation was found between spectral changes and anincrease in CYP1A1 expression measured by the reversetranscriptase polymerase chain reaction (RT-PCR)technique demonstrating that the SR-FTIR method isobserving cellular biochemical changes related to this geneexpression pathway. Finally, the potential use of SR-FTIR spectromicroscopy is discussed as a diagnostic toolfor monitoring cellular exposure and early molecularresponses to environmental pollutants.