pH-Dependent Structures of an i-Motif DNA in Solution

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• 作者：Kyeong Sik Jin<sup>† ; sup><sup>§ ; sup> ; Su Ryon Shin<sup>‡ ; sup><sup>§ ; sup> ; Byungcheol Ahn<sup>† ; sup> ; Yecheol Rho<sup>† ; sup> ; Seon Jeong Kim*<sup>‡ ; sup> ; M
• 刊名：Journal of Physical Chemistry B
• 出版年：2009
• 出版时间：February 19, 2009
• 年：2009
• 卷：113
• 期：7
• 页码：1852-1856
• 全文大小：217K
• 年卷期：v.113,no.7(February 19, 2009)
• ISSN：1520-5207

文摘

We have investigated for the first time the structure of i-motif DNA in solution at various pH conditions by using synchrotron small-angle X-ray scattering technique. To facilitate direct structural comparison between solution structures of i-motif DNA at various pH values, we created atomic coordinates of i-motif DNA from a fully folded to unfolded atomic model. Under mild acidic conditions, the conformations for i-motif DNA appeared to be similar to that of the partially unfolded i-motif atomic model in overall shape, rather than the fully folded i-motif atomic model. Collectively, our observations indicate that i-motif DNA molecule is structurally dynamic over a wide pH range, adopting multiple conformations ranging from the folded i-motif structure to a random coil conformation. As the i-motif structure has been used as an important component in nanomachines, we can therefore believe that the structural evidence presented herein will promote the development of future DNA-based molecular-actuator devices.