Illudins C2 and C3 Stimulate Lipolysis in 3T3-L1 Adipocytes and Suppress Adipogenesis in 3T3-L1 Preadipocytes

详细信息    查看全文

• 作者：Sun-Ok Kim ; Krisada Sakchaisri ; Yukihiro Asami ; In-Ja Ryoo ; Soo-Jin Choo ; Ick-Dong Yoo ; Nak-Kyun Soung ; Young Sang Kim ; Jae-Hyuk Jang ; Bo Yeon Kim ; Jong Seog Ahn
• 刊名：Journal of Natural Products
• 出版年：2014
• 出版时间：April 25, 2014
• 年：2014
• 卷：77
• 期：4
• 页码：744-750
• 全文大小：367K
• 年卷期：v.77,no.4(April 25, 2014)
• ISSN：1520-6025

文摘

The secondary metabolites illudins C₂ (1) and C₃ (2), obtained from the culture broth of Coprinus atramentarius, have been shown to possess antimicrobial activity. In the present study, we discovered novel biological activities of 1 and 2 in lipolysis of differentiated 3T3-L1 adipocytes and adipogenesis of 3T3-L1 preadipocytes. Compounds 1 and 2 exhibit a dose-dependent increase in glycerol release and thereby reduce intracellular lipid accumulation. The stimulatory effects of 1 and 2 on lipolysis are prevented by cAMP-dependent protein kinase (PKA) and extracellular signal-regulated kinase (ERK) inhibitors. Compounds 1 and 2 down-regulated perilipin and also affected the mRNA and protein levels of adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL). However, 1 and 2 treatment leads to a significant increase in PKA-mediated phosphorylation of HSL at S563 and S660. In addition, 1 and 2 treatment in 3T3-L1 preadipocytes induces down-regulation of the critical transcription factors, CCAAT/enhancer binding protein 伪 and 尾 (C/EBP伪 and C/EBP尾), and peroxisome proliferator activated receptor 纬 (PPAR纬), which are required for adipogenesis, and accordingly inhibits adipogenesis. These results suggest that 1 and 2 might be useful for treating obesity due to their modulatory effects on fat by affecting adipocyte differentiation and fat mobilization.