Improving the Integrity of Three-Dimensional Vascular Patterns by Poly(ethylene glycol) Conjugation

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• 作者：Sadhana Sharma ; Wei Tan ; and Tejal A. Desai
• 刊名：Bioconjugate Chemistry
• 出版年：2005
• 出版时间：January 2005
• 年：2005
• 卷：16
• 期：1
• 页码：18 - 22
• 全文大小：385K
• 年卷期：v.16,no.1(January 2005)
• ISSN：1520-4812

文摘

Development of functional tissue-engineering constructs may require that multiple cell types beorganized in controlled three-dimensional (3-D) microarchitectures with proper nutrient diffusion andvascularization. In the past few years, a variety of microscale techniques have demonstrated the abilityto control protein and cell attachment in defined patterns. Nevertheless, maintenance of these patternsover time has been a significant challenge due to nonspecific protein adsorption and cell migration.To this end, we have investigated the effectiveness of poly(ethylene glycol) (PEG) thin films inmaintaining the integrity of 3-D cellular patterns, using human umbilical vein endothelial cells(HUVEC) as a model system. These HUVEC constructs were created using extracellular matrix (ECM)-based microfluidic patterning. Our results indicated that PEG-conjugated substrates improve cellpattern integrity as compared to control silicon. The compliance multifactor (a measure of patternintegrity; higher value means lower pattern integrity) was about 3.66 ± 0.29 on day 5 forPEG-conjugated surfaces, compared with 8.23 ± 0.42 for control surfaces ECM-based microfluidicpatterning coupled with stable PEG-conjugated surfaces may serve as a vital tool for vascularizedtissue engineering.