Colorimetric Logic Gates Based on Ion-Dependent DNAzymes

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• 作者：Li Zhang ; Yun-Mei Zhang ; Ru-Ping Liang ; Jian-Ding Qiu
• 刊名：The Journal of Physical Chemistry C
• 出版年：2013
• 出版时间：June 13, 2013
• 年：2013
• 卷：117
• 期：23
• 页码：12352-12357
• 全文大小：385K
• 年卷期：v.117,no.23(June 13, 2013)
• ISSN：1932-7455

文摘

Two kinds of ion-dependent DNAzymes and tailored substrates enable the design of the logic gates (OR, AND, INHIBIT) using Pb²⁺ and Cu²⁺ as inputs; then, a three-input AND logic gate is developed utilizing the unique feature that Hg²⁺ ions interact with the thymine鈥搕hymine (T鈥揟). At first, the substrates are blocked by ion-dependent DNAzymes that include the horseradish peroxidase (HRP)-mimicking DNAzyme sequence. The released product strands then self-assemble into the hemin/G-quadruplex-HRP-mimicking DNAzyme that biocatalyze the formation of a colored product and provide an output signal for the different logic gates. We are able to recognize the logic output signals effortlessly by our naked eyes. It is a simple, economic, and safe approach for the design of a complex multiple-input DNA logic molecular device.