Field-Scale Demonstration of Enhanced MTBE Bioremediation through Aquifer Bioaugmentation and Oxygenation
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- 作者：Joseph P. Salanitro ; Paul C. Johnson ; Gerard E. Spinnler ; Paul M. Maner ; Halina L. Wisniewski ; and Cristin Bruce
- 刊名：Environmental Science & Technology
- 出版年：2000
- 出版时间：October 1, 2000
- 年：2000
- 卷：34
- 期：19
- 页码：4152 - 4162
- 全文大小：232K
- 年卷期：v.34,no.19(October 1, 2000)
- ISSN：1520-5851
文摘
In situ bioaugmentation and biostimulation experimentswere conducted at the USN Hydrocarbon NationalEnvironmental Test Site at Port Hueneme, CA (PH), wherethe dissolved MTBE groundwater plume is over 1500 mlong. Laboratory microcosm experiments prepared with PHgroundwater showed that MTBE was rapidly metabolized(t1/2 
2 weeks) after inoculation with a high-activity MTBE-degrading bacterial consortium (MC-100). Microcosm studiesalso showed that natural ether degraders were presentat PH; however, the rates were 3-5 times slower than withthe bioaugmented treatment. The field pilot test wasconducted to assess the efficacy of creating an MTBEbiobarrier by inoculating with MC-100 and maintaining well-oxygenated conditions. Three test plots located in the MTBE-only portion of the plume included control (no treatment),O2-only (intermittent O2 gas injection), and O2 + bioaugmented(MC-100) zones. Initial MTBE and dissolved oxygen (DO)concentrations in the plots prior to treatment varied from 2to 9 mg/L and 1 mg/L, respectively. DO levels increasedin the O2-only and O2 + MC-100 plots from 5 to >20mg/L within a few weeks of O2 gas injection. MTBE levelsdecreased in the O2-only plot to 0.01-0.1 mg/L after alag period of 186-261 days, indicating the apparent stimulationof naturally occurring ether degraders. In contrast, inthe O2 + MC-100 plot, MTBE concentrations decreasedafter 30 days and throughout the 261-day experiment eventuallyto 0.001-0.01 mg/L. tert-Butyl alcohol (TBA) concentrationsalso declined in the bioaugmented plot to <0.01 mg/L.
2 weeks) after inoculation with a high-activity MTBE-degrading bacterial consortium (MC-100). Microcosm studiesalso showed that natural ether degraders were presentat PH; however, the rates were 3-5 times slower than withthe bioaugmented treatment. The field pilot test wasconducted to assess the efficacy of creating an MTBEbiobarrier by inoculating with MC-100 and maintaining well-oxygenated conditions. Three test plots located in the MTBE-only portion of the plume included control (no treatment),O2-only (intermittent O2 gas injection), and O2 + bioaugmented(MC-100) zones. Initial MTBE and dissolved oxygen (DO)concentrations in the plots prior to treatment varied from 2to 9 mg/L and 1 mg/L, respectively. DO levels increasedin the O2-only and O2 + MC-100 plots from 5 to >20mg/L within a few weeks of O2 gas injection. MTBE levelsdecreased in the O2-only plot to 0.01-0.1 mg/L after alag period of 186-261 days, indicating the apparent stimulationof naturally occurring ether degraders. In contrast, inthe O2 + MC-100 plot, MTBE concentrations decreasedafter 30 days and throughout the 261-day experiment eventuallyto 0.001-0.01 mg/L. tert-Butyl alcohol (TBA) concentrationsalso declined in the bioaugmented plot to <0.01 mg/L.