Lactoferricin-Related Peptides with Inhibitory Effects on ACE-Dependent Vasoconstriction

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• 作者：Jos&eacute ; M. Centeno ; Marí ; a C. Burguete ; Marí ; a Castelló ; -Ruiz ; Marí ; a Enrique ; Salvador Vall&eacute ; s ; Juan B. Salom ; Germá ; n Torregrosa ; Jos&eacute ; F. Marcos ; Enriq
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：July 26, 2006
• 年：2006
• 卷：54
• 期：15
• 页码：5323 - 5329
• 全文大小：86K
• 年卷期：v.54,no.15(July 26, 2006)
• ISSN：1520-5118

文摘

A selection of lactoferricin B (LfcinB)-related peptides with an angiotensin I-converting enzyme (ACE)inhibitory effect have been examined using in vitro and ex vivo functional assays. Peptides that wereanalyzed included a set of sequence-related antimicrobial hexapeptides previously reported and tworepresentative LfcinB-derived peptides. In vitro assays using hippuryl-L-histidyl-L-leucine (HHL) andangiotensin I as substrates allowed us to select two hexapeptides, PACEI32 (Ac-RKWHFW-NH₂)and PACEI34 (Ac-RKWLFW-NH₂), and also a LfcinB-derived peptide, LfcinB_17-31 (Ac-FKCRRWQWRMKKLGA-NH₂). Ex vivo functional assays using rabbit carotid arterial segments showedPACEI32 (both D- and L-enantiomers) and LfcinB_17-31 have inhibitory effects on ACE-dependentangiotensin I-induced contraction. None of the peptides exhibited in vitro ACE inhibitory activity usingbradykinin as the substrate. In conclusion, three bioactive lactoferricin-related peptides exhibit inhibitoryeffects on both ACE activity and ACE-dependent vasoconstriction with potential to modulatehypertension that deserves further investigation.Keywords: Lactoferricin B-related peptides; ACE inhibition; ex vivo functional assay; ACE-dependentvasoconstriction