Study of the Cultivar-Composition Relationship in Sicilian Olive Oils by GC, NMR, and Statistical Methods
文摘
The aim of this research is to find if there is direct evidence relating the fatty acid composition ofolive oils to specific cultivars grown within a well-limited geographical region. To group olive oilsaccording to their own cultivars,13C high-field nuclear magnetic resonance (NMR) and gas chromatography (GC) were used to analyze 60 extra virgin olive oils from the same Italian region(southwestern Sicily) obtained from four monovarietal cultivars. The 13C NMR spectrum providesinformation about glycerol triesters of olive oils, i.e., about the acyl composition of major componentsand about the fatty acids' positional distribution on the glycerol moiety. GC gives the complete fattyacid profile of olive oil samples. Selection of NMR and GC peaks on the basis of their sensitivity tothe different cultivars was performed by using multivariate analysis of variance (MANOVA). Principalcomponent analysis, tree clustering analysis, multidimensional scaling (MDS), and linear discriminantanalysis (LDA) were then performed on the MANOVA-selected peaks. Results obtained from 13CNMR and GC techniques combined with the multivariate statistical procedure are in good agreementand prove the usefulness of fatty acids analysis to group the monovarietal olive oils belonging to thesame cultivars. Grouping of olive oils according to their cultivars occurs for particular 13C resonancesall belonging to fatty chains in the sn 1,3 position of the glycerol moiety.Keywords: Cultivar; extra virgin olive oil; gas chromatography; NMR; statistical analysis