Synthesis and Characterization of Immunogens Based on Calix[4]arene-crown-6 for the Generation of Antibodies Directed Towards Cesium Ions
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- 作者：Samir Safi ; Zouhair Asfari ; Laurence Ehret-Sabatier ; Maurice Leroy ; Agnè ; s Hagè ; ge
- 刊名：Bioconjugate Chemistry
- 出版年：2006
- 出版时间：September 2006
- 年：2006
- 卷：17
- 期：5
- 页码：1346 - 1350
- 全文大小：148K
- 年卷期：v.17,no.5(September 2006)
- ISSN：1520-4812
文摘
With the aim of generating antibodies directed towards cesium, a calix[4]arene-crown-6 in the 1,3-alternateconformation bearing an N-hydroxysuccinimide function was synthesized. The calixarene was coupled to bovineserum albumin using different calixarene/protein ratios, and cesium was loaded. Conjugates were characterizedusing trinitrobenzenesulfonic acid derivatization, matrix assisted laser desorption ionisation-mass spectrometry(MALDI-TOF-MS), and inductively coupled plasma-mass spectrometry (ICP-MS). Targeted lysines weredetermined after enzymatic cleavage followed by MALDI-TOF-MS, and a progressive targeting related to lysinesaccessibility was observed.