Enzyme-Linked Immunosorbent Assay (ELISA) for Detection of Sulfur-Rich Protein (SRP) in Soybeans (Glycine max L.) and Certain Other Edible Plant Seeds

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• 作者：Erin K. Monaghan ; Mahesh Venkatachalam ; Margaret Seavy ; Kirsten Beyer ; Hugh A. Sampson ; Kenneth H. Roux ; Shridhar K. Sathe
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2008
• 出版时间：February 13, 2008
• 年：2008
• 卷：56
• 期：3
• 页码：765 - 777
• 全文大小：2416K
• 年卷期：v.56,no.3(February 13, 2008)
• ISSN：1520-5118

文摘

As a result of methionine deficiency, legume proteins are considered to be incomplete, and therefore there is a need to explore ways to improve legume protein amino acid balance. Using rabbit anti-soybean sulfur-rich protein (SRP) polyclonal antibodies (pAb), sensitive immunoassays (nanogram sensitivity) were developed. The immunoassays detected SRP in all soybean seeds and soybean-based commercial samples examined. In addition, the presence of pAb cross-reactive proteins was detected in certain dry beans and oilseeds. The cross-reactive proteins were isolated using purified IgG-based immunoaffinity column chromatography. Biochemical analyses including N-terminal amino acid sequencing and amino acid composition indicated that the cross-reactive proteins were comparable to soybean SRP. The cross-reactive proteins contained methionine (1.6–2.4 residues/100 residues) and cysteine (2.4–3.6 residues/100 residues), which satisfies the FAO/WHO recommended pattern for sulfur amino acids in both adults and children (2–5 years old). The results suggest the presence of constitutive SRPs in several dry beans and oilseeds.