Application of an Efficient Gene Targeting System Linking Secondary Metabolites to their Biosynthetic Genes in Aspergillus terreus

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• 作者：Chun-Jun Guo ; Benjamin P. Knox ; James F. Sanchez ; Yi-Ming Chiang ; Kenneth S. Bruno ; Clay C. C. Wang
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：July 19, 2013
• 年：2013
• 卷：15
• 期：14
• 页码：3562-3565
• 全文大小：269K
• 年卷期：v.15,no.14(July 19, 2013)
• ISSN：1523-7052

文摘

Nonribosomal peptides (NRPs) are natural products biosynthesized by NRP synthetases. A kusA-, pyrG- mutant strain of Aspergillus terreus NIH 2624 was developed that greatly facilitated the gene targeting efficiency in this organism. Application of this tool allowed us to link four major types of NRP-related secondary metabolites to their responsible genes in A. terreus. In addition, an NRP affecting melanin synthesis was also identified in this species.