Fluorescent Biotin Analogues for Microstructure Patterning and Selective Protein Immobilization

详细信息    查看全文

• 作者：K. Vijaya Krishna ; Subhadip Ghosh ; Bikramjit Sharma ; Leeju Singh ; Saptarshi Mukherjee ; Sandeep Verma
• 刊名：Langmuir
• 出版年：2015
• 出版时间：November 24, 2015
• 年：2015
• 卷：31
• 期：46
• 页码：12573-12578
• 全文大小：355K
• ISSN：1520-5827

文摘

Benzyl substitution on ureido nitrogens of biotin led to manifestation of aggregation-induced emission, which was studied by steady-state fluorescence, microscopy, and TD-DFT, providing a rationale into the observed photophysical behavior. Besides exhibiting solvatochromism, the biotin derivatives revealed emission peaks centered at 鈭?30 and 545 nm, which has been attributed to the 蟺鈥撓€ stacking interactions. Our TD-DFT results also correlate the spectroscopic data and quantify the nature of transitions involved. The isothermal titration calorimetry data substantiates that the binding of the biotin derivatives with avidin are pretty strong. These derivatives on lithographic patterning present a platform for site specific strept(avidin) immobilization, thus opening avenues for potential applications exploiting these interactions. The fluorescent biotin derivatives can thus find applications in cellular biology and imaging.