Quantification of Topological Coupling between DNA Superhelicity and G-quadruplex Formation

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• 作者：Sangeetha Selvam ; Deepak Koirala ; Zhongbo Yu ; Hanbin Mao
• 刊名：Journal of the American Chemical Society
• 出版年：2014
• 出版时间：October 8, 2014
• 年：2014
• 卷：136
• 期：40
• 页码：13967-13970
• 全文大小：333K
• ISSN：1520-5126

文摘

It has been proposed that new transcription modulations can be achieved via topological coupling between duplex DNA and DNA secondary structures, such as G-quadruplexes, in gene promoters through superhelicity effects. Limited by available methodologies, however, such a coupling has not been quantified directly. In this work, using novel magneto-optical tweezers that combine the nanometer resolution of optical tweezers and the easy manipulation of magnetic tweezers, we found that the flexibility of DNA increases with positive superhelicity (蟽). More interestingly, we found that the population of G-quadruplex increases linearly from 2.4% at 蟽 = 0.1 to 12% at 蟽 = 鈭?.03. The population then rapidly increases to a plateau of 23% at 蟽 < 鈭?.05. The rapid increase coincides with the melting of double-stranded DNA, suggesting that G-quadruplex formation is correlated with DNA melting. Our results provide evidence for topology-mediated transcription modulation at the molecular level. We anticipate that these high-resolution magneto-optical tweezers will be instrumental in studying the interplay between the topology and activity of biological macromolecules from a mechanochemical perspective.