Development of a Method for the Genetic Identification of Mussel Species Belonging to Mytilus, Perna, Aulacomya, and Other Genera

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• 作者：Francisco J. Santaclara ; Montserrat Espiñ ; eira ; Ana G. Cabado ; Arrate Aldasoro ; Nerea Gonzalez-Laví ; n ; Juan M. Vieites
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：November 1, 2006
• 年：2006
• 卷：54
• 期：22
• 页码：8461 - 8470
• 全文大小：601K
• 年卷期：v.54,no.22(November 1, 2006)
• ISSN：1520-5118

文摘

Legislation regarding the labeling of processed products is an important issue in the protection ofconsumer rights. This labeling is especially important in products that cannot be identified on thebasis of their morphological characters, because these are removed from the animal in thetransformation process. The goal of this study was the identification of mussel species usingPolymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) and Forensically Informative Nucleotide Sequencing (FINS) methodologies. The molecular marker selected was18S rDNA (nuclear small-subunit rDNA gene), which allows identification at the genus level and atthe species level in some cases. The genera included in this study were Mytilus, Perna, Aulacomya,Semimytilus, Brachidontes, Choromytilus, and Perumytilus. Different markers were used for geneticidentification at the species level. To identify the species included in the genus Perna and Choromytilus,a fragment of ITS 1 (Internal Transcribed Spacer 1) was amplified by multiplex PCR and digestedwith restrictases. The species of Mytilus were identified by length polymorphism and RFLP of thepolyphenolic adhesive protein gene. This methodology was validated with products manufactured inthe authors' pilot plant and applied to commercial samples. Therefore, this sequential method canbe completely or partially used to determine the mussel genus or species present in any food product.