Detection of Land Animal Remains in Fish Meals by the Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Technique

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• 作者：Francisco J. Santaclara ; Montserrat Espiñ ; eira ; Ana G. Cabado ; Juan M. Vieites
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2007
• 出版时间：January 24, 2007
• 年：2007
• 卷：55
• 期：2
• 页码：305 - 310
• 全文大小：241K
• 年卷期：v.55,no.2(January 24, 2007)
• ISSN：1520-5118

文摘

In the present study a technique was developed with the aim of guaranteeing the composition andsecurity of fish meals, since it allows verification of whether these meals contain land animal remains.The method is based on polymerase chain reaction (PCR) and length polymorphism, followed by arestriction fragment length polymorphism (RFLP). Specific primers for every species were designedand calibrated, generating exclusively a PCR product with a specific size when DNA for each specieswas present in the sample. This technique allows the detection of land animal remains in fish meals,specifically cow, chicken, pig, horse, sheep, and goat. The identity of the PCR products can beconfirmed by RFLP analysis using only one restriction enzyme. The selected restrictase generatedone characteristic restriction profile for every species included in this study. The detection limit ofthis method was calculated by using mixtures of fish meals in different proportions and meal thatexclusively contained remains of one of these land species studied. The analytical strategy hereinproposed was applied to fish and meat meals, giving good results, both in the analyzed standardsand in commercial samples.