In Situ Microscopy for In-line Monitoring of the Enzymatic Hydrolysis of Cellulose

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• 作者：Britta Opitz ; Andreas Prediger ; Christian L眉der ; Marrit Eckstein ; Lutz Hilterhaus ; Patrick Lindner ; Sascha Beutel ; Thomas Scheper ; Andreas Liese
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：September 3, 2013
• 年：2013
• 卷：85
• 期：17
• 页码：8121-8126
• 全文大小：361K
• 年卷期：v.85,no.17(September 3, 2013)
• ISSN：1520-6882

文摘

A new in-line method for the monitoring of enzymatic hydrolysis of cellulose is described. Using a new in situ microscope prototype, the noninvasive determination of particle size distributions was possible. For the automated analysis of the acquired images, a new processing algorithm called CelluloseAnalyzer was developed. It enabled tracking of the number of particles and moreover allowed monitoring of the proportions of particle size fractions during the course of enzymatic hydrolysis reactions. Using this technique, significant differences between hydrolysis with endoglucanases and cellulase mixtures were observed. Furthermore, the in situ microscopy results were compared with results from off-line measurements with laser diffraction spectroscopy and gel permeation chromatography.