Mechanisms of Patulin Toxicity under Conditions That Inhibit Yeast Growth

详细信息    查看全文

• 作者：Yumiko Iwahashi ; Hiroshi Hosoda ; Ji-Hyun Park ; Joo-Hee Lee ; Yoshiteru Suzuki ; Emiko Kitagawa ; Satomi Mizukami Murata ; Nam-Soo Jwa ; Man-Bock Gu ; and Hitoshi Iwahashi
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2006
• 出版时间：March 8, 2006
• 年：2006
• 卷：54
• 期：5
• 页码：1936 - 1942
• 全文大小：384K
• 年卷期：v.54,no.5(March 8, 2006)
• ISSN：1520-5118

文摘

Patulin, 4-hydroxy-4H-furo[3,2c]pyran-2(6H)-one, is one of the best characterized and most widelydisseminated mycotoxins found in agricultural products. Nonetheless, the mechanisms by which patulincauses toxicity are not well understood. Thus, the cytotoxicity of patulin was characterized by analysisof the yeast transcriptome upon challenge with patulin. Interestingly, patulin-induced yeast geneexpression profiles were found to be similar to gene expression patterns obtained after treatmentwith the antifungal agricultural chemicals thiuram, maneb, and zineb. Moreover, patulin treatmentwas found to activate protein degradation, especially proteasome activities, sulfur amino acidmetabolism, and the defense system for oxidative stress. Damage to DNA by alkylation was alsosuggested, and this seemed to be repaired by recombinational and excision repair mechanisms.Furthermore, the results provide potential biomarker genes for the detection of patulin in agriculturalproducts. The results suggest the possibility of applying the yeast transcriptome system for theevaluation of chemicals, especially for natural chemicals that are difficult to get by organic synthesis.Keywords: Patulin; DNA microarry; yeast; mutagenesis; mycotoxin