Synthesis and Properties of 2鈥?O,4鈥?C-Ethyleneoxy Bridged 5-Methyluridine

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• 作者：Yoshiyuki Hari ; Tomohiko Morikawa ; Takashi Osawa ; Satoshi Obika
• 刊名：Organic Letters
• 出版年：2013
• 出版时间：July 19, 2013
• 年：2013
• 卷：15
• 期：14
• 页码：3702-3705
• 全文大小：238K
• 年卷期：v.15,no.14(July 19, 2013)
• ISSN：1523-7052

文摘

2鈥?O,4鈥?C-Ethyleneoxy bridged 5-methyluridine (EoNA-T), possessing a seven-membered linkage and an anomeric 4鈥?carbon, was synthesized and introduced into oligonucleotides by using an automated DNA synthesizer. The EoNA-modified oligonucleotides significantly stabilized the duplexes with single-stranded RNA and triplexes with double-stranded DNA relative to the natural oligonucleotide and oligonucleotides modified by another seven-membered bridged 5-methyluridine, 2鈥?4鈥?BNA^COC-T. In addition, EoNA-T showed excellent nuclease resistance.