Engineered Polymer-Supported Synthesis of 3鈥?Carboxyalkyl-Modified Oligonucleotides and Their Applications in the Construction of Biochips for Diagnosis of the Diseases

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• 作者：Satyakam Patnaik ; Sandip K. Dash ; Dalip Sethi ; Ashok Kumar ; K. C. Gupta ; Pradeep Kumar
• 刊名：Bioconjugate Chemistry
• 出版年：2012
• 出版时间：March 21, 2012
• 年：2012
• 卷：23
• 期：3
• 页码：664-670
• 全文大小：287K
• 年卷期：v.23,no.3(March 21, 2012)
• ISSN：1520-4812

文摘

An engineered polymer support 5 has been prepared for the solid-phase assembly of 3鈥?carboxyalkyl-modified oligonucleotides using commonly available reagents. A two-step deprotection procedure resulted in the quantitative cleavage of oligonucleotides from the support and removal of the protecting groups from phosphodiesters and exocyclic amino groups of the nucleic bases. The fully deprotected oligomers, obtained in high yield, were desalted and analyzed on RP-HPLC. After characterization by MALDI-TOF, these carboxyalkylated oligonucleotides were immobilized onto the epoxy-functionalized glass microslides to prepare biochips. The performance of these biochips was evaluated under different sets of conditions and then successfully validated by the detection of base mismatches and human infectious disease, bacterial meningitis, caused by N. meningitidis.