Identification of a Minimal Peptide Tag for in Vivo and in Vitro Loading of Encapsulin

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• 作者：Caleb Cassidy-Amstutz ; Luke Oltrogge ; Catherine C. Going ; Antony Lee ; Poh Teng ; David Quintanilla ; Alexandra East-Seletsky ; Evan R. Williams ; David F. Savage
• 刊名：Biochemistry
• 出版年：2016
• 出版时间：June 21, 2016
• 年：2016
• 卷：55
• 期：24
• 页码：3461-3468
• 全文大小：418K
• 年卷期：0
• ISSN：1520-4995

文摘

The encapsulation of enzymes and other proteins within a proteinaceous shell has been observed in many bacteria and archaea, but the function and utility of many such compartments are enigmatic. Efforts to study these functions have been complicated by the size and complexity of traditional protein compartments. One potential system for investigating the effect of compartmentalization is encapsulin, a large and newly discovered class of protein shells that are typically composed of two proteins: a protomer that assembles into the icosahedral shell and a cargo protein packaged inside. Encapsulins are some of the simplest known protein shell systems and readily self-assemble in vivo. Systematic characterization of the effects of compartmentalization requires the ability to load a wide range of cargo proteins. Here, we demonstrate that foreign cargo can be loaded into the encapsulin from Thermotoga maritima both in vivo and in vitro by fusion of the cargo protein with a short C-terminal peptide present in the native cargo. To facilitate biochemical characterization, we also develop a simple and rapid purification protocol and demonstrate the thermal and pH stability of the shell. Efforts to study the biophysical effects of protein encapsulation have been problematic in complex compartments, but the simplicity of assembling and loading encapsulin makes it an ideal system for future experiments exploring the effects of encapsulation on proteins.