Structural Analysis of HopPmaL Reveals the Presence of a Second Adaptor Domain Common to the HopAB Family of Pseudomonas syringae Type III Effectors

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• 作者：Alex U. Singer ; Bin Wu ; Adelinda Yee ; Scott Houliston ; Xiaohui Xu ; Hong Cui ; Tatiana Skarina ; Maite Garcia ; Anthony Semesi ; Cheryl H. Arrowsmith ; Alexei Savchenko
• 刊名：Biochemistry
• 出版年：2012
• 出版时间：January 10, 2012
• 年：2012
• 卷：51
• 期：1
• 页码：1-3
• 全文大小：194K
• 年卷期：v.51,no.1(January 10, 2012)
• ISSN：1520-4995

文摘

HopPmaL is a member of the HopAB family of type III effectors present in the phytopathogen Pseudomonas syringae. Using both X-ray crystallography and solution nuclear magnetic resonance, we demonstrate that HopPmaL contains two structurally homologous yet functionally distinct domains. The N-terminal domain corresponds to the previously described Pto-binding domain, while the previously uncharacterised C-terminal domain spans residues 308鈥?85. While structurally similar, these domains do not share significant sequence similarity and most importantly demonstrate significant differences in key residues involved in host protein recognition, suggesting that each of them targets a different host protein.