Chiroptical Effects in the Second Harmonic Signal of Collagens I and IV

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• 作者：Ana-Maria Pena ; Thierry Boulesteix ; Thibault Dartigalongue ; and Marie-Claire Schanne-Klein
• 刊名：Journal of the American Chemical Society
• 出版年：2005
• 出版时间：July 27, 2005
• 年：2005
• 卷：127
• 期：29
• 页码：10314 - 10322
• 全文大小：397K
• 年卷期：v.127,no.29(July 27, 2005)
• ISSN：1520-5126

文摘

We performed polarization-resolved surface second harmonic generation (SHG) experimentson thin films of collagen I and IV molecules, as well as conventional CD measurements. We found thatcollagen IV presents little CD and no SHG optical activity, whereas collagen I exhibits large chiropticaleffects involving both one-electron and excitonic coupling mechanisms. We estimated that these chiralcomponents enhance the SHG signal from fibrillar collagen in biological tissues by typically a factor of 2.By comparing the distinct behaviors of collagens I and IV in SHG microscopy and in surface SHGexperiments, we concluded that SHG microscopy is a sensitive probe of the micrometer-scale structuralorganization of collagen in biological tissues.