Toward a Prostate Specific Antigen-Based Prostate Cancer Diagnostic Assay: Preparation of Keyhole Limpet Hemocyanin -Conjugated Normal and Transformed Prostate Specific Antigen Fragments

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• 作者：Vadim Y. Dudkin ; Justin S. Miller ; Anna S. Dudkina ; Christophe Antczak ; David A. Scheinberg ; Samuel J. Danishefsky
• 刊名：Journal of the American Chemical Society
• 出版年：2008
• 出版时间：October 15, 2008
• 年：2008
• 卷：130
• 期：41
• 页码：13598-13607
• 全文大小：702K
• 年卷期：v.130,no.41(October 15, 2008)
• ISSN：1520-5126

文摘

Prostate specific antigen (PSA) molecules secreted by cancerous and normal prostate cells differ in their N-linked glycan composition, while the peptide backbone appears to be conserved. Antibodies selectively recognizing such differentially glycosylated PSA structures could form a basis for a new diagnostic assay for prostate cancer. Twenty-amino acid PSA fragments carrying di-, tri-, and tetrabranched complex-type glycans were prepared by total synthesis and conjugated to maleimide-modified keyhole limpet hemocyanin (KLH) carrier protein through backbone Cys residues. These glycopeptide/KLH conjugates were then used for antibody generation.