Deploying RNA and DNA with Functionalized Carbon Nanotubes
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- 作者：Simone Alidori ; Karim Asqiriba ; Pablo Londero ; Magnus Bergkvist ; Marco Leona ; David A. Scheinberg ; Michael R. McDevitt
- 刊名：The Journal of Physical Chemistry C
- 出版年：2013
- 出版时间：March 21, 2013
- 年：2013
- 卷：117
- 期：11
- 页码：5982-5992
- 全文大小：480K
- 年卷期：v.117,no.11(March 21, 2013)
- ISSN：1932-7455
文摘
Carbon nanotubes internalize into cells and are potential molecular platforms for siRNA and DNA delivery. A comprehensive understanding of the identity and stability of ammonium-functionalized carbon nanotube (f-CNT)-based nucleic acid constructs is critical to deploying them in vivo as gene delivery vehicles. This work explored the capability of f-CNT to bind single- and double-strand oligonucleotides by determining the thermodynamics and kinetics of assembly and the stoichiometric composition in aqueous solution. Surprisingly, the binding affinity of f-CNT and short oligonucleotide sequences was in the nanomolar range, kinetics of complexation were extremely rapid, and from one to five sequences were loaded per nanotube platform. Mechanistic evidence for an assembly process that involved electrostatic, hydrogen bonding, and 蟺-stacking bonding interactions was obtained by varying nanotube functionalities, oligonucleotides, and reaction conditions. 31P NMR and spectrophotometric fluorescence emission data described the conditions required to assemble and stably bind a DNA or RNA cargo for delivery in vivo and the amount of oligonucleotide that could be transported. The soluble oligonucleic acid鈥揻-CNT supramolecular assemblies were suitable for use in vivo. Importantly, key evidence in support of an elegant mechanism by which the bound nucleic acid material can be 鈥渙ff-loaded鈥?from the f-CNT was discovered.